Nephrology Dialysis Transplantation, Vol 12, Issue 11 2258-2262, Copyright © 1997 by Oxford University Press
P Arrizabalaga, M Sole, I Quinto and C Ascaso
Background: Mononuclear leucocytes have a role in IgA
nephropathy (IgAN). Renal leucocyte recruitment is mediated by adhesive
interactions between leucocytes and their ligands on renal cells.
Methods: We have assessed interstitial and glomerular
leucocytes by avidin-biotin-peroxidase with monoclonal antibodies (MA)
against leucocytes (CD45), {beta}2-integrin (CD18), monocyte-macrophages
(CD14), T (CD3) and T-cell subsets (CD4, CD8), and intercellular adhesion
molecule 1 (ICAM-1) (CD54), and analysed their relation with the abnormal
expression of ICAM-1 on proximal tubule epithelium in sequential renal
sections from 48 patients with IgAN stratified according to the severity of
the interstitial cellular infiltration observed by light microscopy.
Results: In IgAN without (n=15) and with (n=7)
interstitial cellular infiltration of 1+, ICAM-1 expression on vascular
endothelium was unchanged with respect to that observed in the normal
kidney; the proximal tubule epithelium was negative for this stain. In IgAN
with interstitial cellular infiltration of 2+ (n=10), 3+ (n=11), and 4+
(n=5), ICAM-1+ stain was observed on the proximal
tubule epithelium, the median value of its quantitative expression being
0.3, 0.1, and 0.2 (P=0.0008), respectively. The tubular
ICAM-1+ stain was significantly associated with the
interstitial leucocytes identified by MA, and correlated with
CD45+ (r=0.59, P=0.02), CD14+
(r=0.54, P<0.02), and CD3+ (r=0.51, P=0.02)
interstitial leucocytes in IgAN with interstitial cellular infiltration.
Interstitial ICAM-1+ and
CD18+ leucocytes were correlated (r=0.56,
P<0.001). Correlation was found between the quantitative tubular
expression of ICAM-1+ and the number of
CD45+ (r=0.98, P<0.0001),
CD3+ (r=0.48, P=0.02), and
CD8+ (r=0.76, P<0.02) glomerular leucocytes.
Conclusion: Our results suggest that tubular and
interstitial ICAM-1+ cells may participate in
adhesive interactions with interstitial leucocytes. Interstitial T-cells
and macrophages as well as glomerular T-cells bearing predominantly
CD8+ phenotype could play a role in the induction of
the tubular expression of ICAM-1 in IgAN. Key words:
IgA nephropathy; interstitial and glomerular leucocyte infiltration;
intercellular adhesion molecule-1
ORIGINAL ARTICLES
Intercellular adhesion molecule-1 mediated interactions and leucocyte infiltration in IgA nephropathy
Services of Nephrology and Pathology, and Epidemiological and Biostatistical Unit, Hospital Clinic I Provincial, University of Barcelona, Spain; Corresponding author at: Servei de Nefrologia, Hospital Clinic I Provincial, c/Villarroel, 170, E-08036 Barcelona, Spain
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