Nephrology Dialysis Transplantation, Vol 12, Issue 10 2064-2068, Copyright © 1997 by Oxford University Press
M Sharma, J Li, R Sharma, M Artero, X Ge, E McCarthy, H Wang and V Savin
Background: Tripterygium
wilfordii Hook F is a medicinal plant used for the treatment of
glomerulonephritis in China. We studied the effect of
Tripterygium wilfordii multiglycoside (TWG) on
glomerular albumin permeability (Palbumin) in vitro.
Method: Isolated rat glomeruli were incubated with
protamine (600 &mgr;g/ml) for 30 min, or with human recombinant tumour
necrosis factor (TNF-&agr;, 0.4 ng/ml), superoxide (10 units/ml), or
serum from a focal segmental glomerular sclerosis (FSGS) patient for 10 min
at 37°C. TWG, 1 mg/ml, was added in parallel tubes to study the
effect on Palbumin. Control glomeruli were incubated under identical
conditions. The albumin reflection coefficient (&sgr;albumin) was
calculated from the change in glomerular volume in response to an applied
oncotic gradient. Convectional permeability (Palbumin) was calculated as (1
- &sgr;albumin). Results: Compared with controls,
protamine increased the Palbumin of glomeruli (0.83±0.05, n=25
vs 0.18±0.03, n=20); pretreatment with TWG blocked this effect
(0.13±0.04, n=25). TNF-&agr; also increased the Palbumin
(0.79±0.04, n=24 vs 0.04±0.07, n=19); preincubation
blocked this effect (0.03±0.09, n=24). Palbumin of glomeruli
incubated with xanthine and xanthine oxidase, resulting in the production
of superoxide, also increased as compared to controls
((0.85±0.04, n=15 vs 0.08±0.05, n=14); TWG blocked
this effect as well (0.21±0.08, n=14). FSGS serum also increased
Palbumin of glomeruli significantly (0.88±0.02, n=49 vs
0.00±0.02, n=49); preincubation with TWG blocked this effect
(0.05±0.07, n=30). TWG by itself had no effect on Palbumin
(0.19±0.01, n=15). Conclusion: Our results
show that TWG blocks protamine, TNF-&agr;, superoxide, and FSGS
serum-mediated increase in glomerular albumin permeability in
vitro. We conclude that reduction of proteinuria by
Tripterygium wilfordii multiglycoside in various kinds
of glomerular diseases in vivo might be due to
protection of the glomerular filtration barrier.
Keywords: protamine; tumour necrosis factor-&agr;;
superoxide; focal segmental glomerular sclerosis; albumin permeability;
Tripterygium wilfordii Hook F
ORIGINAL ARTICLES
Inhibitory effect of Tripterygium wilfordii multiglycoside on increased glomerular albumin permeability in vitro
Department of Medicine, Division of Nephrology, Medical College of Wisconsin, Milwaukee, Wisconsin, USA; Institute of Nephrology, Beijing Medical University, Beijing, China; Istituto di Medicina Clinica, University of Trieste, Trieste, Italy; Corresponding author at: Froedtert Memorial Lutheran Hospital, 9600 West Wisconsin Avenue, Milwaukee, Wisconsin 53226, USA
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  M. Sharma, R. Sharma, E. T. McCarthy, and V. J. Savin The Focal Segmental Glomerulosclerosis Permeability Factor: Biochemical Characteristics and Biological Effects Experimental Biology and Medicine, January 1, 2004; 229(1): 85 - 98. [Abstract] [Full Text] [PDF]
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