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Nephrology Dialysis Transplantation, Vol 12, Issue 1 51-56, Copyright © 1997 by Oxford University Press

ORIGINAL ARTICLES

Alternative pathway complement activation induces proinflammatory activity in human proximal tubular epithelial cells

S David, L Biancone, C Caserta, B Bussolati, V Cambi and G Camussi
Cattedra di Nefrologia, Facolta di Medicina e Chirurgia, Universita di Parma, Parma, Italy; Cattedra di Nefrologia, II Facolta di Medicina e Chirurgia, Universita di Pavia, Varese, Italy; Corresponding author at: Laboratorio di Immunopatologia, Istituto di Nefro-Urologia, C.so Dogliotti 14, 10126 Torino, Italy

Background. Proximal tubular epithelial cells express a surface C3-convertase activity which induces C fixation and insertion of the C5b-9 membrane attack complex (MAC) into the cell plasma membrane. The physiopathological consequences of this phenomenon are unknown. Methods. The effect of C fixation on the production of inflammatory mediators by human proximal tubular epithelial cells in culture was explored. Results. Proximal tubular epithelial cells incubated with a sublytic amount of normal human serum as a source of C, but not with heat-inactivated human serum, showed a time-dependent calcium influx and a concomitant release of 14C-arachidonic acid (14C-AA). Eicosanoid synthesis following the arachidonic acid mobilization was studied as prostaglandin E2 release. Mg2+/EGTA, which did not prevent C activation by the C3-convertase, and p-bromodiphenacyl bromide, a phospholipase A2-inhibitor, inhibited mobilization of 14C-AA. These results suggest the activation of an extracellular Ca2+-dependent, phospholipase A2. Complement fixation was associated with the synthesis of proinflammaotry cytokines such as IL-6 and TNF-&agr;. Experiments with C6-deficient sera indicated that the release of 14C-AA and the production of cytokines were dependent on the insertion of the terminal components of complement in the plasma membrane. Indeed, the reconstitution of normal haemolytic activity of C6-deficient sera with purified C6 restored also the release of 14C-AA and the production of cytokines. Conclusion. In vitro complement activation on the proximal tubular cell surface triggers the generation of proinflammatory mediators, which may potentially contribute to the pathogenesis of tubulointerstitial injury. Keywords: complement; cytokine; memrane attack complex; proximal tubule; tubular injury
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