Skip Navigation

This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (3)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Romero, M.
Right arrow Articles by Rodriguez-Iturbe, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Romero, M.
Right arrow Articles by Rodriguez-Iturbe, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nephrology Dialysis Transplantation, Vol 12, Issue 1 174-179, Copyright © 1997 by Oxford University Press

ORIGINAL ARTICLES

A simple method to identify NBT-positive cells in isolated glomeruli

M Romero, J Mosquera and B Rodriguez-Iturbe
Instituto de Investigaciones Clinicas, Universidad del Zulia, Venezuela; Renal Service, Hospital Universitario and Instituto de Investigaciones Biomedicas (INBIOMED), Maracaibo, Venezuela; Corresponding author at: Unidad de Dialisis y Trasplante Renal, Hospital Universitario (9 piso), Aparado Postal 1430, Maracaibo, 4001-A, Venezuela

Background. Reactive oxygen radicals are probably involved in the pathogenesis of human and experimental models of renal disease, yet current methods are inadequate to quantify and identify the cells producing reactive oxygen radicals. Methods and Results. We used the nitroblue tetrazolium reaction to determine superoxide anion production in glomerular cells in phorbol myristate-stimulated glomerular suspensions and in isolated glomeruli from rats with nephrotoxic nephritis, ureteral obstruction, and puromycin aminonucleoside nephrosis. We were also able to identify these nitroblue tetrazolium+cells using specific appropriate antibodies. When the technique was tested in conditions known to increase reactive oxygen radicals, as phorbol myristate-stimulated glomeruli and glomeruli from animals with nephrotoxic nephritis and ureteral obstruction, increased number of nitroblue tetrazolium+cells were found. These cells were identified as glomerular intrinsic cells (Thy-1 +) or infiltrating leukocytes (leukocyte common antigen+ or antineutrophil+). Conclusions. This method may be useful to determine cells participating in glomerular damage induced by reactive oxygen radicals. Keywords: glomerulus; nitroblue tetrazolium; reactive oxygen radicals
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
C. R. Bonnett, E. J. Cornish, A. G. Harmsen, and J. B. Burritt
Early Neutrophil Recruitment and Aggregation in the Murine Lung Inhibit Germination of Aspergillus fumigatus Conidia
Infect. Immun., December 1, 2006; 74(12): 6528 - 6539.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.