Nephrol Dial Transplant (1996) 11: 608-613
© 1996 European Renal Association-European Dialysis and Transplant Association
research-article
Daily short exposure of cultured mesothelial cells to lactated, high-glucose, low-pH peritoneal dialysis fluid induces a low-profile regenerative steady state
Department of Nephrology and the Kornach Laboratory for Experimental Nephrology, Central Emek Hospital Afula, Israel
Correspondence and offprint requests to: Correspondence and offprint requests to: L. Gotloib, MD, Dept. of Nephrology, Central Emek Hospital, Afula 18101, Israel
This study was designed to evaluate cytotoxic effects and influence upon cell growth of cultured mesothelial cells exposed to modified 4.25% Dianeal dialysate fluid (M-199 in Dianeal solution, glucose 4.25 g, pH 5.2) (Expr. group), 60 min a day for a total period of follow-up of 13 consecutive days, compared with that observed in a control group (C).
Beginning on day 7, the cell counts in group C were significantly higher than those observed at zero time (P<0.05). Cell counts in the experimental group showed no significant differences between the first day of culture and each one of the 13 consecutive days of follow-up.
Thymidine incorporation into DNA observed on the first day in C, was significantly higher (P<0.01) beginning on the 10th day. Values observed in the experimental group were low during the whole period of follow-up. LDH mean values at each time interval, were significantly higher (P<0.01 and <0.001) for cells exposed to the dialysis solution.
Repeated exposure of the mesothelium to 40 mMol/l lactate and high glucose concentrations induced severe cell injury and death, decreased cell growth and, consequently, a reduced rate of regeneration which is extended as long as the repeated exposure is maintained.
Keywords: biocompatibility of dialysis solutions; mesothelial cells culture