Nephrol Dial Transplant (1995) 10: 2244-2249
© 1995 European Renal Association-European Dialysis and Transplant Association
research-article
In-vivo induction of monocyte chemotactic and activating factor in patients with chronic renal failure
Departments of Internal Medicine and Clinical Pathology, Kitasato University School of Medicine; Sagami Junkanki Clinic
Correspondence and offprint requests to: Correspondence and offprint requests to: Tohru Akahoshi MD, Department of Internal Medicine, Kitasato University School of Medicine, 1-15-1 Kitasato, Sagamihara, Kanagawa 228, Japan.
BACKGROUND: Monocyte chemotactic and activating factor (MCAF) is a novel inflammatory cytokine belonging to the chemokine superfamily and stimulates chemotaxis and activation of monocytes. Increased production of inflammatory cytokines has been shown in patients with end-stage renal disease (ESRD). This study was thus conducted to determine plasma MCAF in patients with ESRD.
METHODS: Plasma levels of MCAF were determined by ELISA. Gene expression of MCAF in PBMC was assessed by RT-PCR followed by southern blot hybridization.
RESULTS: Plasma MCAF in 72 patients with long-term haemodialysis (HD) (162.4 ± 58.2 pg/ml) and eight uraemic patients not yet dialysed (167.6 ± 57.7 pg/ml) was found to exceed significantly the level in 24 normal subjects (86.0±19.4 pg/ml). MCAF before HD session in long-term HD patients was the same whether HD was carried out with either cellulosic (CUP) or synthetic (PMMA) membrane dialysers. Intradialytic increase in plasma MCAF during a single HD session was observed in both patient groups dialysed with CUP or PMMA membranes. The results of RT-PCR analysis indicated that haemodialysis stimulates the gene expression of MCAF in PBMC in vivo.
CONCLUSIONS: The present results indicate that increased levels of plasma MCAF may promote the activation of monocytes in patients with ESRD.
Keywords: chronic renal failure; haemodialysis; monocyte chemotactic and activating factor
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